1. Konami Logo
2. Princess Melora
3. Dr. Warmon's Ambition
4. Twinbee to the Rescue!
5. Option Select Fanfare
6. Character Select
7. Next Destination
8. Green Hills ~ Blue Skies
9. Sunset Over the Hills
10. Let's Move On!
11. How Did We Do
12. Crazy Attack!
13. We're Done Here!
14. All Clear!
15. Prism Caverns
16. Deeper Into the Caverns
17. Pastel Forest
18. Fast Flowing Rivers
19. Danger Approaches
20. A Child's Fantasy Realm
21. Distorted Fantasy
22. World of Ice and Snow
23. Rainbow Music Factory
24. Run! Run!
25. Evil Twin bee
26. Celebration
27. Peace Has Returned
28. Staff Roll
29. Game Over
30. Ten Seconds
31. May the Best Bee Win
32. Last Bee Standing
33. Pause Sound
34. Rainbow Bell Obtained