This sound is from Spy Soundboard - Team Fortress 2

Team Fortress 2 is a 2007 multiplayer first-person shooter game developed and published by Valve Corporation. It is the sequel to the 1996 Team Fortress mod for Quake and its 1999 remake, Team Fortress Classic.
See also: Spy (disambiguation), Team Fortress Classic, 2Fort, John Patrick Lowrie, Spy Hard, The Orange Box.