This sound is from Step Brothers Soundboard

Step Brothers is a 2008 American comedy film directed by Adam McKay and written by Will Ferrell and McKay. It follows Brennan (Ferrell) and Dale (Reilly) two grown men who are forced to live together as brothers after their single parents marry each other.
See also: Step Brothers, G-Eazy, The Four Step Brothers, Don Trip, Step Brothers (duo), and Stepsibling.