This sound is from Wheatley Voice Lines

Voicelines of everyone favourite british core Wheatley. Wheatley is a spherical AI designed to dampen GLaDOS's intelligence, but he is ultimately more mischievous than he is intelligent. He is quick to make friends with Chell, but he is also quick to betray her for his own gain. In the end, Wheatley is defeated by Chell and GLaDOS, but he leaves a lasting impression on players as one of the most memorable characters in the Portal franchise.