This sound is from Ladies And Gentlemen Soundboard

Ladies and Gentlemen may refer to ladies and gentlemen (salutation) The phrase is a common introductory phrase. Ladies and gentlemen may also refer to Ladies and Gentleman (salutations), a common phrase for women and gentlemen. The term is used to refer to women and men who are in the middle of their lives.
See also: Ladies & Gentlemen (2015 film), Ladies and Gentlemen We Are Floating in Space, And Now... Ladies and Gentlemen, Ladies and Gentlemen, The Fabulous Stains, Ladies and gentlemen (salutation), and Ladies vs Gentlemen.