This sound is from Day Of The Tentacle Soundboard

Day of the Tentacle is a 1993 graphic adventure game developed and published by LucasArts. A remastered version of the game was released in March 2016, for Windows, OS X, Linux, PlayStation 4 and PlayStation Vita.
See also: Maniac Mansion, Tentacle erotica, Tim Schafer, LucasArts adventure games, List of video games considered the best, and Dave Grossman (game developer).