This sound is from The Pick Of Destiny Soundboard

Another album, More Rocktastic Music from the Film, contains some small excerpts of the official score that the soundtrack excludes. The Pick of Destiny is the band's first studio release in five years, following up their self-titled debut album in 2001.
See also: Tenacious D in The Pick of Destiny, Tenacious D, Pick of Destiny, Jack Black, List of songs recorded by Tenacious D, and Colin Hanks.