This sound is from Ed Helms Soundboard

Edward Parker Helms is an American actor and comedian. He has received six Screen Actors Guild Award nominations for Outstanding Performance by an Ensemble in a Comedy Series winning in 2008. Helms has also starred in dramatic films and comedic films such as Cedar Rapids, Jeff Who Lives at Home, Vacation and Vacation.
See also: Tag (2018 film), Ed Harris, The Hangover, Jesse Helms, We're the Millers, and The Hangover Part II.