This sound is from Mass Effect 2: Tali'Zorah nar Rayya (vas Normandy) Soundboard

~ Tali'Zorah vas Normandy to Shepard
Tali'Zorah nar Rayya (vas Normandy) is one of the main protagonists of the original Mass Effect video game trilogy. She is a quarian who Shepard originally meets when she is on her Pilgrimage and becomes a member of the Normandy crew. Tali holds the distinction of being one of only two squadmates available in all three games, the other being Garrus Vakarian. She is voiced by Ash Sroka (credited as Liz Sroka in the first two games).