This sound is from Mass Effect 2: Jeff "Joker" Moreau Soundboard

Flight Lieutenant Jeff "Joker" Moreau is the pilot of the SSV Normandy. Joker is an excellent pilot and confident to the point of arrogance, wasting no time in pointing out his prowess to others. He's quick to ensure everyone knows his many accomplishments and commendations were all earned, and not due to charity for his medical problems.