This sound is from Firefly Soundboard

Firefly is an American space Western drama television series which ran from 2002–2003, created by writer and director Joss Whedon, under his Mutant Enemy Productions label. Whedon served as an executive producer, along with Tim Minear. The series is set in the year 2517, after the arrival of humans in a new star system and follows the adventures of the renegade crew of Serenity, a "Firefly-class" spaceship. Starring: Nathan Fillion, Gina Torres, Alan Tudyk, Morena Baccarin, Adam Baldwin, Jewel Staite, Sean Maher, Summer Glau and Ron Glass.