This sound is from GLaDOS Soundboard

GLaDOS (Genetic Lifeform and Disk Operating System) is a fictional artificially superintelligent computer system from the video game series Portal. The inspiration for the character's creation extends from Wolpaw's use of a text-to-speech program while writing lines for Psychonauts.
See also: List of Portal characters, Portal (video game), Ellen McLain, Portal (series), Wheatley (Portal), Potato Sack.