This sound is from Scary Movie 2 (2001)

"Scary Movie 2," released in 2001, is a hilarious parody film that spoofs various horror movies. Directed by Keenen Ivory Wayans, the ensemble cast includes Anna Faris, Marlon Wayans, Shawn Wayans, Regina Hall, and Chris Masterson. The movie takes place in a haunted mansion where a group of college students are tasked with staying the night for a psychological experiment. Full of slapstick comedy and movie references, this outrageous sequel will have you laughing non-stop. Play and download the sounds of this hilarious movie here.