This sound is from A Million Ways to Die in the West (2014)

"A Million Ways to Die in the West" is a 2014 movie directed by Seth MacFarlane. It follows the story of a cowardly cowboy in the Old West who must overcome his fear to win back his girlfriend. This hilarious comedy Western stars Seth MacFarlane himself, alongside Charlize Theron, Liam Neeson, Amanda Seyfried, and Neil Patrick Harris. If you're intrigued by the sounds of this film, you can play and download them here.