This sound is from Ed - Ed, Edd n Eddy: The Mis-Edventures - Ed Voices (PlayStation 2)

Ed, Edd n Eddy The Mis-Edventures is a 3D platform video game based on the Cartoon Network animated television series Ed, Ed d Eddy.
See also: Ed, Edd n Eddy, List of Ed, Edd n Eddy characters, Ed, Edd n Eddy: Scam of the Century, Ed, Edd n Eddy: Jawbreakers!, Sam Vincent (voice actor), Peter Kelamis.