This sound is from O Brother, Where Art Thou? (2000)

O Brother, Where Art Thou? is a highly acclaimed film directed by the Coen brothers and released in 2000. Set in Depression-era Mississippi, it follows three escaped convicts, Ulysses Everett McGill (played by George Clooney), Pete Hogwallop (played by John Turturro), and Delmar O'Donnell (played by Tim Blake Nelson), on their quest for hidden treasure. The movie brilliantly combines comedy, adventure, and musical elements.

The soundtrack of O Brother, Where Art Thou? is phenomenal, featuring traditional folk and bluegrass music from the 1930s. It showcases remarkable artists like Alison Krauss, Emmylou Harris, Gillian Welch, the Soggy Bottom Boys (a fictional band led by Clooney's character), and many more. You can experience the soul-stirring sounds of this film by playing and downloading the soundtrack here.