This sound is from Hot Rod (2007)

Hot Rod is a hilarious comedy film released in 2007, directed by Akiva Schaffer. The movie stars an ensemble cast including Andy Samberg, Jorma Taccone, and Bill Hader. It follows the misadventures of Rod Kimble, an amateur stuntman who aspires to jump over a row of buses on his moped to raise money for his stepfather's life-saving surgery. Filled with quirky characters, slapstick humor, and outrageous stunts, Hot Rod is a cult classic that never fails to entertain. If you're in the mood for laughter, you can play and download the sounds of this unforgettable film here.