This sound is from The House Bunny (2008)

The House Bunny is a 2008 American comedy film directed by Fred Wolf. Starring Anna Faris as Shelley Darlingson, a Playboy Bunny who is evicted from the Playboy Mansion, the film follows her misadventures as she becomes the house mother of a misfit sorority. The cast also includes Colin Hanks, Emma Stone, Kat Dennings, and Hugh Hefner in a cameo appearance. With its hilarious storyline and charming performances, The House Bunny delivers a delightful blend of humor and heart. If you're in the mood for some laughter, you can play and download the sounds of this film right here.