This sound is from Step Brothers (2008)

Step Brothers (2008) is a comedy film directed by Adam McKay. Starring Will Ferrell and John C. Reilly as Brennan and Dale, two middle-aged men who become stepbrothers when their parents marry, the film follows their hilarious and often absurd misadventures as they try to coexist under one roof. This uproarious movie is known for its outrageous humor and over-the-top performances. Complete with laugh-out-loud moments and unforgettable scenes, Step Brothers is a must-watch comedy. You can play and download the movie here.