This sound is from Blazing Saddles (1974)

Blazing Saddles, a comedy film released in 1974, directed by Mel Brooks, is a hilarious and satirical take on the western genre. It follows Bart, a black sheriff's appointee, and his attempts to save a town from the schemes of corrupt officials. Starring Cleavon Little as Bart and Gene Wilder as Jim, the film also features memorable performances by Madeline Kahn, Harvey Korman, and Slim Pickens. Experience the laughter with the iconic sounds of Blazing Saddles by playing and downloading them here.